Use of immunoglobulin-loaded protein A-bearing staphylococci as a primary solid phase immunoadsorbent in radioimmunoassay.

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Use of immunoglobulin-loaded protein A-bearing staphylococci as a primary solid phase immunoadsorbent in radioimmunoassay.

Protein A-bearing formalinized heat-inactivated Staphylococcus aureus bind rabbit 125I-IgG with high capacity of IgG for antigen. The affinity of immobilized IgG for antigen is equal to the affinity of soluble antibody, and the capacity for antigen approximates the capacity of soluble antibody for antigen. 125I-IgG bound to high affinity sites on bacteria is not substantially displaced in the p...

متن کامل

Solid-phase radioimmunoassay with protein-A-bearing Staphylococcus aureus cells used to assay a protein (ferritin) and a hapten (digoxin).

We compared use of protein-A-containing Staphylococcus aureus bacteria with conventional ammonium sulfate precipitation and second-antibody methods of separating bound and free antigen in the radioimmunoassay of a hapten (digoxin) and protein (ferritin) in human sera. In each case, values obtained with the heat-killed, formalin-fixed bacteria correlated well with those found by established meth...

متن کامل

the significance of conjunction as a cohesive device in teaching writing

the research questions were as follows: 1. is there any relationship between the students concious awareness of the form and implications of the conjuncations and their improvement in using appropriate conjunctions? 2. does students knowledge of the from and the implications of the conjunctions help them to produce more coherent writings. 3. does a comparison between english conjunctions and th...

15 صفحه اول

the effectiveness of strategy-based instruction in teaching english as a second or foreign language: a meta-analysis of experimental studies

a large number of single research studies on the effects of strategy-based instruction (sbi) in teaching english as a foreign or second language has been conducted so far. however, the lack of a comprehensive meta-analysis targeting the effectiveness of english language sbi is observed. moreover, the findings of experimental studies regarding the context of the english language, proficiency lev...

Staphylococcal enterotoxins A and B: solid-phase radioimmunoassay in food.

An immunoassay employing (125)I labeled enterotoxins A and B and polystyrene tubes coated with specific antibodies was used for detection and quantitation of enterotoxin in food. Ham salad, cheddar cheese, custard, condensed milk, and salami were studied. Enterotoxin was successfully determined in all the foods by simple extraction procedures. The assay was sensitive to 1 to 10 ng of toxin per ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1980

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(19)86212-9